Ethanol Production From Cassava Wastes (Pulp And Peel) Using Alcohol Tolerant Yeast Isolated From Palm Wine

E J Archibong, Ifeanyi E Obika, O I Okafor, U I Okafor, C S Ezewuzie, C C Ezemba, N C Awah, B C Okeke, G C Anaukwu, V N Anakwenze


Introduction: The efficient ethanol production using Saccharomyces spp. isolated from palm wine in utilizing industrial wastes (cassava pulp and peel) was studied in the liquid state fermentation process. Methods: The percentage alcohol in the cassava pulp and peel was obtained by digesting the cassava waste with three different commercial exogenous enzymes which include alpha amylase (Termamyl), Amyloglucosidase (AMG), β-glucanase t a-amylase (Cereflo) and fermenting with yeast (Saccharomyces spp.) isolated from palm wine. The combination of two enzymes in starch hydrolysis produced more sugars than individual enzyme usage and also yielded more of ethanol when compared to a single enzyme activity. It was also observed that AMG combined with Termamyl yielded 2.05% ethanol. Cereflo combined with Termamyl yielded 1.6% ethanol while Tennamyl alone yielded 1.26% ethanol from cassava pulp. However, it was also observed that AMG combined with Termamyl yielded 0.46% ethanol, Cereflo combined with Termamyl yielded 0.73% ethanol while Termamyl alone yielded 0.33% ethanol from cassava peel.

Results: This implies that ethanol produced from cassava pulp is higher than ethanol produced from cassava peel, since the cassava pulp contains high starch than the peel.

Conclusions: These digestions using these enzymes and subsequent ethanol production can go a long way in waste management for economic purposes.


Ethanol; Manihot; Amylases; Glucan 1,4-alpha-Glucosidase

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