Presence of Bacteria With Pathogenic Potential Among Already-Used Toothbrushes From University Students

Arthur Chinedu Okafor, Serah N Igwesi, Elijah E David, Vitus K Okolo, Kingsley Agu

Abstract


Introduction: The most common oral hygiene aid used to improve the oral health of an individual is the toothbrush. Hence, it could play a role in initiation and transmission of infections and diseases. This study aimed at investigating the presence of haemolytic bacteria on used toothbrushes.

Methods: A close-ended questionnaire was designed and distributed to a hundred Nigerian students. This was employed to ascertain the attitudes of students towards their toothbrush. Twenty samples of already-used toothbrush (1-3 months) were randomly collected from students. The heads containing bristles were detached soaked in peptone water and vortexed. Serial dilution was done and appropriate dilution factor was plated out on Nutrient agar medium. The isolates were tentatively identified with morphological and biochemical properties. They were also assayed for haemolytic activity and antibiotics susceptibility.

Results: Results indicated that 80% of the students responded to the questionnaire, out of which > 60% agreed to have sometime injured themselves while brushing their teeth. A little above half of the participants do not care about the purity of the water they use in brushing. Majority (70%) saw no use submitting their toothbrush for microbiological assessment. However, 81.25% replace their toothbrush within 5 months of use. Seventeen out of the twenty samples examined had aerobic bacterial count of > 104 cfu/toothbrush. The 24 isolates obtained were found to belong to Lactobacillus, Staphylococcus, Streptococcus, and Bacillus genera with 55%, 30%, 20%, and 15% occurrence, respectively. Moreover, 40% of the isolates were found to be either alpha or beta haemolytic indicating their potential to cause disease.

Conclusions: Generally, all isolates were susceptible to most of the commonly used antibiotics. It is envisaged that the results of this study would chaperone oral hygiene practice among individuals such as the immunocompromised.

Keywords


Oral Hygiene; Streptococcus; Lactobacillus

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References


Downes J, Hooper SJ, Wilson MJ, Wade WG. Prevotella histicola sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol. 2008;58(Pt 8):1788-91. DOI: 10.1099/ijs.0.65656-0 PMID: 18676457

Karibasappa GN, Nagesh L, Sujatha BK. Assessment of microbial contamination of toothbrush head: an in vitro study. Indian J Dent Res. 2011;22(1):2-5. DOI: 10.4103/0970-9290.79965 PMID: 21525668

Glass RT, Lare MM. Toothbrush contamination: a potential health risk? Quintessence Int. 1986;17(1):39-42. PMID: 3457393

Quirynen M, de Soete M, Pauwels M, Goossens K, Teughels W, van Eldere J, et al. Bacterial survival rate on tooth- and interdental brushes in relation to the use of toothpaste. J Clin Periodontol. 2001;28(12):1106-14. PMID: 11737507

Mbakwem–Aniebo C, Odoemelam HA, Okonko IO. Isolation and Identification of Candida albicans and Staphylococcus aureus from Oral Swabs among Primary School Pupils in Uzuakoli, Abia State, Nigeria. Nat Sci. 2012;10:9-16.

Vinogradov AM, Winston M, Rupp CJ, Stoodley P. Rheology of biofilms formed from the dental plaque pathogen Streptococcus mutans. Biofilms. 2004;1(01):49-56.

Fernandes VH, Cesar DL, editors. Microbiology evaluation of toothbrushes. In vitro Cellular and Developmental Biology of Animal; 2006: SOCIETY IN VITRO BIOLOGY 514 DANIELS STREET, STE 411, RALEIGH, NC 27605 USA.

Boylan R, Li Y, Simeonova L, Sherwin G, Kreismann J, Craig RG, et al. Reduction in bacterial contamination of toothbrushes using the Violight ultraviolet light activated toothbrush sanitizer. Am J Dent. 2008;21(5):313-7. PMID: 19024257

Folaranmi N, Akaji E, Onyejaka N. Pattern of presentation of oral health conditions by children at University of Nigeria Teaching Hospital, Enugu: a retrospective study. Niger J Clin Pract. 2014;17(1):47-50. DOI: 10.4103/1119-3077.122836 PMID: 24326807

Jain N, Mitra D, Ashok KP, Dundappa J, Soni S, Ahmed S. Oral hygiene-awareness and practice among patients attending OPD at Vyas Dental College and Hospital, Jodhpur. J Indian Soc Periodontol. 2012;16(4):524-8. DOI: 10.4103/0972-124X.106894 PMID: 23493177

Woolgrove J, Cumberbatch G, Gelbier S. Understanding dental attendance behaviour. Community Dent Health. 1987;4(3):215-21. PMID: 3477305

Hamilton ME, Coulby WM. Oral health knowledge and habits of senior elementary school students. J Public Health Dent. 1991;51(4):212-9. PMID: 1941772

Peker I, Akarslan Z, Basman A, Haciosmanoglu N. Knowledge and behavior of dentists in a dental school regarding toothbrush disinfection. Braz Oral Res. 2015;29:48. DOI: 10.1590/1807-3107BOR-2015.vol29.0048 PMID: 25789506

Taji SS, Rogers AH. ADRF Trebitsch Scholarship. The microbial contamination of toothbrushes. A pilot study. Aust Dent J. 1998;43(2):128-30. PMID: 9612987

American Dental Association (ADA). ADA statement on toothbrush care: cleaning, storage and replacement 2009 [July 9, 2015]. Available from: www.ada.org/1887.aspx.

Eisler L, Wearda K, Romatoski K, Odland RM. Morbidity and cost of odontogenic infections. Otolaryngol Head Neck Surg. 2013;149(1):84-8. DOI: 10.1177/0194599813485210 PMID: 23585157

Centre for Disease Control and Prevention (CDC). Dental hygiene 2009 [July 9, 2015]. Available from: www.cdc.gov/healthywater/hygiene/dental/

Jiang H, Petersen PE, Peng B, Tai B, Bian Z. Self-assessed dental health, oral health practices, and general health behaviors in Chinese urban adolescents. Acta Odontol Scand. 2005;63(6):343-52. PMID: 16512107

Dilip CL. Health status, treatment requirements, knowledge and attitude towards oral health of police recruits in Karnataka. J Indian Assoc Pub Health Dent. 2005;5(5):20.

Al-Shammari KF, Al-Ansari JM, Al-Khabbaz AK, Dashti A, Honkala EJ. Self-reported oral hygiene habits and oral health problems of Kuwaiti adults. Med Princ Pract. 2007;16(1):15-21. DOI: 10.1159/000096134 PMID: 17159358

Efstratiou M, Papaioannou W, Nakou M, Ktenas E, Vrotsos IA, Panis V. Contamination of a toothbrush with antibacterial properties by oral microorganisms. J Dent. 2007;35(4):331-7. DOI: 10.1016/j.jdent.2006.10.007 PMID: 17118507

Verran J, Leahy-Gilmartin AA. Investigations into the microbial contamination of toothbrushes. Microbios. 1996;85(345):231-8. PMID: 8699965

Eichenauer J, von Bremen J, Ruf S. Microbial contamination of toothbrushes during treatment with multibracket appliances. Head Face Med. 2014;10:43. DOI: 10.1186/1746-160X-10-43 PMID: 25301033

Nishikawara F, Katsumura S, Ando A, Tamaki Y, Nakamura Y, Sato K, et al. Correlation of cariogenic bacteria and dental caries in adults. J Oral Sci. 2006;48(4):245-51. PMID: 17220624

Balashova NV, Crosby JA, Al Ghofaily L, Kachlany SC. Leukotoxin confers beta-hemolytic activity to Actinobacillus actinomycetemcomitans. Infect Immun. 2006;74(4):2015-21. DOI: 10.1128/IAI.74.4.2015-2021.2006 PMID: 16552030




DOI: http://dx.doi.org/10.21859/ajlsr-040302

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