Assessing of salt effect in regulation of growth and antioxidant defence in Arthrocnemum indicum under Arsenic stress alone or with salinity

Dhouha Belhadj SGHAIER


Metal pollution is one of the most acute environmental issues, aggravated by human activities. In the present study, authors intend to determine the tolerance traits of Arthrocnemum indicum to avoid As toxicity. In order to evaluate the mechanisms responsible for Arsenic (As) tolerance, plants were grown in semi-controlled conditions upon exposure to different As concentrations (0, 200, 500 and 800 µM) supplemented or not with NaCl (0, 200 mM). Growth parameters, micronutrient uptake, proline and glycine betaine contents and antioxidant enzymes activities (SOD, GPX and APX) were assessed. Plants demonstrated a good growth even after prolonged exposure to high metal concentrations. On the other hand, the GB and malondialdehyde content in the leaves of stressed plants increased significantly in all treatments. However, proline showed a steady level. Activities of SOD, GPX and APX varied according to the applied stress. Our results indicated that As induced oxidative stress in Arthrocnemum indicum and enzymatic and non-enzymatic antioxidants played significant roles in tolerance to As toxicity.


Arsenic, combined stress, mineral uptake, antioxidant mechanisms

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Khlifi R, Olmedo P, Gil F, Feki-Tounsi M, Hammami B, Rebai A, Hamza-Chaffai A. Biomonitoring of cadmium, chromium, nickel and arsenic in general population living near mining and active industrial areas in Southern Tunisia. Environ Monit Assess. 2014; 186:761-79.

Alsahli AA, Bhat JA, Alyemeni MN, Ahmad P. Hydrogen Sulfide (H2S) Mitigates Arsenic (As)-Induced Toxicity in Pea (Pisum sativum L.) Plants by Regulating Osmoregulation, Antioxidant Defense System, Ascorbate Glutathione Cycle and Glyoxalase System. J Plant Growth Regul. 2021; 40:2515-2531.

Alam MR, Rahman MM, Tam NFY, Man Kit Yu R, MacFarlane GR, The accumulation and distribution of arsenic species and selected metals in the saltmarsh halophyte, spiny rush (Juncus acutus). Mar Pollut Bull. 2022; 175:113373. 2022.113373.

Hasanuzzaman M, Nahar K, Alam MM, Bhowmik PC, Hossain MA, Rahman MM, Prasad MNV, Ozturk M, Fujita M. Potential use of halophytes to remediate saline soils. Biomed Res Internat. 2014; 589341: 1-12.

Nisar F, Gul B, Ajmal Khan M, Hameed A. Germination and recovery responses of heteromorphic seeds of two co-occurring Arthrocnemum species to salinity, temperature and light. S Afr J Bot. 2019; 121:143-151. j.sajb.2018.10.035.

Sghaier DB, Duarte B, Bankaji I, Caçador I, Sleimi N. Growth, chlorophyll fluorescence and mineral nutrition in the halophyte Tamarix gallica cultivated in combined stress conditions: Arsenic and NaCl, J Photochem Photobiol B. 2015; 149:204-214. 2015.06.003

Sghaier DB, Bankaji I, Pedro S, Caçador I, Sleimi N. Photosynthetic Behaviour and mineral nutrition of Tamarix gallica cultivated under Aluminum and NaCl combined stress. Phyton. 2019; 88:239-252. https://

Hewitt EJ. Sand and water culture methods used in the study of plant nutrition, J Assoc Off Anal Chem. 1966; 49: 888-889.

Tiryakioglu M, Eker S, Ozkutlu F, Husted S, Cakmak I. Antioxidant defense system and cadmium uptake in barley genotypes differing in cadmium tolerance. J Trace Elem Med Biol. 2006; 20: 181-189. https://doi.10.1016/j.jtemb.2005.12. 004.

Bergmeyer HU, Gawehn K, Grassl M. Enzymes as biochemical reagents. In: Bergmeyer HU (Ed) Methods in enzymatic analysis. Academic Press, New York. 1974; 515-517.

Marklund S, Marklund G. Involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47: 469-474. https://doi.10.1111/ j.1432-1033.1974.tb03714.x.

Bradford M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dyebinding. Anal Biochem. 1976; 72: 248-254. https://doi.10.1016/0003-2697 (76)90527-3.

Heath RL, Packer L. Photoperoxidation in isolated chloroplasts I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys. 1968; 125:189-198. https://doi.10.1016/0003-9861 (68)90654-1.

Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant Soil. 1973; 39: 205-207.

Grieve CM, Grattan SR. Rapid assay for determination of water soluble quaternary ammonium compounds. Plant Soil. 1983; 70: 303-307 BF02374789.

Ahmad P, Alyemeni MN, Al-Huqail AA, Alqahtani MA, Wijaya L, Ashraf M, Kaya C, Bajguz A. Zinc Oxide Nanoparticles Application Alleviates Arsenic (As) Toxicity in Soybean Plants by Restricting the Uptake of as and Modulating Key Biochemical Attributes, Antioxidant Enzymes, Ascorbate-Glutathione Cycle and Glyoxalase System. Plants. 2020; 9: 825. 10.3390/plants9070825.

Nikalje GC, Suprasanna P. Coping with metal toxicity – cues from halophytes. Front Plant Sci. 2018; 9:777.

Sarath NG, Shackira AM, El-Serehy HA, Hefft DI, Puthur JT. Phytostabilization of arsenic and associated physio-anatomical changes in Acanthus ilicifolius L. Environ Pollut. 2022; 298:118828.

Patel M, Parida AK. Salinity alleviates the arsenic toxicity in the facultative halophyte Salvadora persica L. by the modulations of physiological, biochemical, and ROS scavenging attributes. J Hazard Mater. 2020; 401: 123368.

Panda A, Rangani J, Kumari A, Parida A K. Efficient regulation of arsenic translocation to shoot tissue and modulation of phytochelatin levels and antioxidative defense system confers salinity and arsenic tolerance in the Halophyte Suaeda maritima. Environ Exp Bot. 2017; 143: 149-171.

Cheng M, Wang A, Liu Z, Gendall AR, Rochfort S, Tang C. Sodium chloride decreases cadmium accumulation and changes the response of metabolites to cadmium stress in the halophyte Carpobrotus rossii. Ann Bot. 2018; 122: 373-385.

Parvez S, Abbas G, Shahid M, Amjad M, Hussain M, Asad SA, et al. Effect of salinity on physiological, biochemical and photostabilizing attributes of two genotypes of quinoa (Chenopodium quinoa Willd.) exposed to arsenic stress. Ecotoxicol Environ Saf. 2020; 187: 109814.

Abbas G, Murtaza B, Bibi I, Shahid M, Niazi NK, Khan MI, et al. Arsenic uptake, toxicity, detoxification, and speciation in plants: physiological, biochemical, and molecular aspects. Int J Environ Res Public Health. 2018; 15: 59.

Parida AK, Veerabathini SK, Kumari A, Agarwal PK. Physiological, Anatomical and Metabolic Implications of Salt Tolerance in the Halophyte Salvadora persica under Hydroponic Culture Condition. Front Plant Sci. 2016; 7:351. 3389/fpls.2016.00351

Vromman D, Lefèvre I, Šlejkovec Z, Martínez JP, Vanhecke N, Briceño M, et al. Salinity influences arsenic resistance in the xerohalophyte Atriplex atacamensis Phil. Environ Exp Bot. 2016; 126 : 32-43.

Petrov V, Hille J, Mueller-Roeber B, Gechev TS. ROS-mediated abiotic stress-induced programmed cell death in plants. Front Plant Sci. 2015; 18: 69.

Zhou MX, Renard ME, Quinet M, Lutts S. Effect of NaCl on proline and glycinebetaine metabolism in Kosteletzkya pentacarpos exposed to Cd and Zn toxicities. Plant Soil. 2019; 441: 525-542.

Kofroňová M, Hrdinová A, Mašková P, Tremlová J, Soudek P, Petrová Š, et al. Multi-Component Antioxidative System and Robust Carbohydrate Status, the Essence of Plant Arsenic Tolerance. Antioxidants. 2020; 9: 283. 10.3390/ antiox9040283.

Kumari A, Goyal V, Sheokand S. Oxidative Stress and Antioxidant Defence Under Metal Toxicity in Halophytes. In: Hasanuzzaman M, Nahar K, Öztürk M. (eds) Ecophysiology, Abiotic Stress Responses and Utilization of Halophytes. Springer, Singapore. 2019.

Sruthi P, Puthur JT. Cadmium stress alleviation potential of Bruguiera cylindrica (L.) Blume enhances in combination with NaCl. Bioremediation J. 2021; 100038:1-24. 10.1080/10889868.2021.1911923.


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