Reno-protective and Membrane Stabilizing Effect of Dioscorea bulbifera L. in CCL4 Induced Toxicity in Rats

U. Subasini, S. Thenmozhi G.Victor Rajamanickam, G.P.Dubey and Sumeet Dwivedi


The Hydroalcoholic extract of Dioscorea bulbifere L was evaluated for its reno-protective and membrane stabilizing effect against CCl4 induced toxicity in Rats. Animals were divided into four groups. First group was considered to be of normal, whereas second group meant for CCl4, third and fourth groups were kept for CCl4 and different doses of extract. The treatment was continued for 21 days. On 22nd day, animals were sacrificed and analyzed for the levels of various biochemical parameters such as Na + K + ATPase, Ca 2+ ATPase, Mg 2+ ATP ase, aspartate transaminase, alanine transaminase, alkaline phosphatase, acid phosphatase, catalase, glutathione–s-transferase, reduced glutathione and malondialdehyde. Alkaline phosphatase, acid phosphatase, aspartate transaminase and alanine transaminase were decreased significantly while antioxidants like catalase, glutathione-s-transferase and reduced glutathione were remained the other way. Membrane stabilizing enzymes like Na+, K+, ATPase, Ca2+ ATPase, Mg2+ and ATPase were increased significantly in treated animals. These observations suggest that hydroalocholic extract of Dioscorea bulbifera L at a dose of 400 mg/kg, b.wt as an effective dose for treating CCl4 intoxicated rats.

Keywords: CCl4, membrane stabilizing effect, Dioscorea bulbifera, reno-protection

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