Evaluation of Anti-angiogenic Activities of Aqueous Extracts of Regular and Selenium-rich Green Teas Using Chick Chorioallantoic Membrane as an Experimental Model

Abdul-Lateef Molan, Wen-Hua Wei, Janyawat Vuthijumnonk


Angiogenesis, a process of developing new blood vessels, is essential in tumor progression and metastasis. It is well received that consumption of green tea (Camellia sinensi) can reduce risk of cancers to some extent. Here we used the chick chorioallantoic membrane (CAM) as a model system to study any anti-angiogenic effects generated by crude water extract of regular green tea (R-GTE) and selenium-rich green tea (Se-GTE). Comparing to the control CAM treated with only distilled water, the CAMs treated with the same volume of crude extracts of both R-GTE and Se-GTE had much less new blood vessels formed, indicating clear anti-angiogenic effects by the green teas. Se-GTE was superior to R-GTE and this superiority was attributed to the presence of higher level of organic selenium in comparison to the R-GTE. The anti-angiogenic properties of water extracts of both R-GTE and Se-GTE could be clues to the reduced risk of coronary heart diseases and cancers following consumption of green tea reported in numerous studies.


antiangiogenic effect, chick chorioallantoic membrane (CAM), regular green tea, selenium-rich green tea.

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