ABNORMAL PRESENTATION OF SERUM MICRO-NUTRIENTS IN WISTAR RATS: CONSEQUENCE OF VIRGIN ENGINE OIL EXPOSURE

Ayobola Abolape Iyanda

Abstract


Very many harmful practices are found in Africa. The use of engine oil for various therapeutic and cosmetics purposes, is one of them. Petroleum products are made up of toxic components; therefore the effects of virgin oil on micro-nutrient levels are being investigated.  30 rats divided equally into 5 groups were named GROUPS 1-5. GROUP 1, GROUP 2, GROUP 3, and GROUP 4 were treated with 0.5, 0.5, 1.0, and 1.0 mg/kg body weight of engine oil respectively. GROUP 1 and GROUP 3 were administered by oral-route while GROUP 2 and GROUP 4 were dosed through the dermal-route. GROUP 5 served as the control. On the 31st day, blood samples were obtained from the rats, centrifuged and the resultant serum stored at - 20ºC until the time they were utilized for analysis of vitamins (High Performance Liquid Chromatography); as well as Mg and trace elements (Atomic Absorption Spectrometry). P≤ 0.05 was considered significant. Significant differences were observed for folic acid, Mg, Mn, and Zn as well as vitamins A, C, E, and D irrespective of dose and route of administration. These results suggest that exposure to engine oil can alter micro-nutrient metabolism.

 


Keywords


virgin engine oil; magnesium; micro-nutrients; dermal and oral routes.

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DOI: http://dx.doi.org/10.21859/ajlsr-05024

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